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RAMP2 Antibody (PA5-47846) in WB
Western blot analysis of RAMP2 in D3 mouse embryonic stem cell line. Samples were incubated in RAMP2 polyclonal antibody (Product # PA5-47846) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for RAMP2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47846
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse RAMP2 Ser45-Val159
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576471
Product Specific Information
In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) RAMP2 is observed, and less than 1% cross-reactivity with rhRAMP1 is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTMUSP00000002615; OTTMUSP00000037624; Receptor activity-modifying protein 2; RP23-281C18.6
Gene Aliases: Ramp2
UniProt ID: (Mouse) Q9WUP0
Entrez Gene ID: (Mouse) 54409
angiogenesis
vasculogenesis
sprouting angiogenesis
cAMP biosynthetic process
transport
calcium ion transport
intracellular protein transport
signal transduction
G-protein coupled receptor signaling pathway
heart development
regulation of blood pressure
regulation of G-protein coupled receptor protein signaling pathway
positive regulation of gene expression
protein transport
positive regulation of cAMP biosynthetic process
receptor internalization
adherens junction assembly
cellular response to vascular endothelial growth factor stimulus
negative regulation of vascular permeability
positive regulation of angiogenesis
bicellular tight junction assembly
basement membrane assembly
protein localization to plasma membrane
vascular smooth muscle cell development
negative regulation of endothelial cell apoptotic process
positive regulation of vasculogenesis
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