Invitrogen

RAN Monoclonal Antibody (ARAN1)

RAN Antibody in Immunocytochemistry (ICC/IF)

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RAN Antibody (MA1-20581) in ICC/IF

Immunofluorescence analysis of RAN was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAN Monoclonal Antibody (ARAN1) (Product # MA1-20581) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilutio... View More

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RAN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAN siRNA and decrease in signal intensity was observed in western blot application using Anti-RAN Monoclonal Antibody (Product # MA1-20581). {KD}

产品信息

MA1-20581

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Bovine, Hamster, Human, Mouse, Rat, Xenopus

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

ARAN1

抗原

Denatured, recombinant, human ran protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.3 mg/mL

纯化类型

purified

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_560865

产品详细信息

Recommended positive controls: Jurkat.

靶标信息

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress.

Disease models & mechanisms

Cho KI,Yi H,Tserentsoodol N,Searle K,Ferreira PA

MA1-20581 was used in immunocytochemistry to investigate the neuroprotective effect of RANBP2 insufficiency against light damage

Mon Dec 27 00:00:00 UTC 2010

生物信息学

蛋白别名: Androgen receptor-associated protein 24; ARA25; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; OTTHUMP00000166078; OTTHUMP00000166102; ran GTP-binding protein; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein

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基因别名: ARA24; Gsp1; I79_024397; OK/SW-cl.81; RAN; ran-1; RanTC4; Rasl2-8; TC4; XELAEV_18007741mg; xran