Invitrogen

RANBP10 Polyclonal Antibody

RANBP10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RANBP10 Antibody (PA5-117680) in IHC (P)

Immnohistochemistry (Paraffin) analysis of RANBP10 in paraffin-embedded human skeletal muscle tissue using RANBP10 Polyclonal Antibody (Product # PA5-117680) diluted at 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The... View More

RANBP10 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-117680

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫沉淀 (IP)

1:200-1:2,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Ran-binding protein 10 protein (3-165aa)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.11 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2902309

产品详细信息

This antibody has been tested in direct-ELISA

靶标信息

The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.

仅用于科研。不用于诊断过程。未经明确授权不得转售。