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RAP80 Antibody (PA5-96617) in ICC/IF
Immunofluorescence analysis of RAP80 in GFP-RNF168 transgenic U2OS cells. Samples were incubated with RAP80 Polyclonal antibody (Product # PA5-96617). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
产品信息
PA5-96617
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UIMC1 (NP_0573743)
偶联物
形式
Liquid
浓度
0.10 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808419
产品详细信息
Immunogen sequence: MPRRKKKVKE VSESRNLEKK DVETTSSVSV KRKRRLEDAF IVISDSDGEE PKEENGLQKT KTKQSNRAKC LAKRKIAQMT EEEQFALALK MSEQEAREVN SQEEEEEELL RKAIAESLNS CRPSDASATR SRPLATGPSS QSHQEKTTDS
Positive Samples: 293T, HeLa, NCI-H460, Mouse intestine, Rat brain; Cellular Location: Nucleus
靶标信息
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRCA1-A complex subunit RAP80; Receptor-associated protein 80; retinoid X receptor interacting protein 110; Retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1
基因别名: 9430016E08Rik; D330018D10Rik; D630032M02Rik; RAP80; RGD1307009; RIP110; RXRIP110; UIMC1; X2HRIP110
UniProt ID: (Human) Q96RL1, (Mouse) Q5U5Q9, (Rat) Q5PQK4
Entrez Gene ID: (Human) 51720, (Mouse) 20184, (Rat) 290997
double-strand break repair
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
response to ionizing radiation
G2 DNA damage checkpoint
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
histone H2A K63-linked deubiquitination
DNA repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
chromatin modification
