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RAPGEF2 Antibody (PA5-69195) in IHC (P)
Immunohistochemical analysis of RAPGEF2 in paraffin-embedded human adrenal tissue. Sample was probed with a RAPGEF2 polyclonal antibody (Product # PA5-69195) at a dilution of 1:200. Detection was performed with a donkey anti-Rabbit Cy3 secondary antibody at a dilution of 1:200. Images were taken at 20x magnification with an exposure time of 0.5-2.0 seconds.
产品信息
PA5-69195
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
synthetic peptide directed towards the C-terminal region of mouse RAPGEF2
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 2% sucrose
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2688335
产品详细信息
This target displays homology in the following species: Cow: 79%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 100%; Rat: 100%
靶标信息
Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CNrasGEF; Cyclic nucleotide ras GEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA(Ras/Rap1A-associating)-GEF; RA-GEF-1; Rap guanine nucleotide exchange factor (GEF) 2; Rap guanine nucleotide exchange factor 2; Ras/Rap1-associating GEF-1
基因别名: 5830453M24Rik; CNRasGEF; KIAA0313; mKIAA0313; nRap GEP; NRAPGEP; PDZ-GEF1; PDZGEF1; RA-GEF; RA-GEF-1; Rap-GEP; RAPGEF2
UniProt ID: (Human) Q9Y4G8
Entrez Gene ID: (Human) 9693, (Mouse) 76089
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
GTPase activator activity
calcium ion binding
protein binding
Rap guanyl-nucleotide exchange factor activity
diacylglycerol binding
PDZ domain binding
cAMP binding
cGMP binding
beta-1 adrenergic receptor binding
WW domain binding
phosphatidic acid binding
guanyl-nucleotide exchange factor activity
protein kinase binding
guanyl-nucleotide exchange factor
MAPK cascade
blood vessel development
neuron migration
G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
small GTPase mediated signal transduction
negative regulation of cell proliferation
positive regulation of neuron projection development
cAMP-mediated signaling
ventricular system development
forebrain neuron development
microvillus assembly
neuron projection development
brain-derived neurotrophic factor receptor signaling pathway
positive regulation of protein binding
Rap protein signal transduction
intracellular signal transduction
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of cAMP-mediated signaling
positive regulation of protein kinase activity
negative regulation of melanin biosynthetic process
regulation of synaptic plasticity
negative regulation of dendrite morphogenesis
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
cellular response to cGMP
adenylate cyclase-activating adrenergic receptor signaling pathway
protein localization to plasma membrane
establishment of endothelial intestinal barrier
regulation of cell junction assembly
cellular response to nerve growth factor stimulus
positive regulation of cAMP-dependent protein kinase activity
positive regulation of dendritic cell apoptotic process
positive regulation of vasculogenesis
positive regulation of neuron migration
signal transduction
multicellular organism development
nervous system development
cell differentiation
