Invitrogen

RASA1 Monoclonal Antibody (B4F8)

RASA1 Antibody in Immunocytochemistry (ICC/IF)

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RASA1 Antibody (MA4-001) in ICC/IF

Immunofluorescent analysis of GTPase Activating Protein (green) showing positive staining in the cytoplasm of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a GTPase Activating Protein monoclonal antibody (Product # MA4-001) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were was... View More

Product Image Gallery

RASA1 Antibody — IP-MS enrichment of RASA1 (LFQ intensity): RASA1 was enriched 137-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and RASA1 antibody (Product # MA4-001). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

产品信息

MA4-001

应用

建议稀释比

已发表文章

免疫印迹 (WB)

10 µg/mL

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免疫细胞化学 (ICC/IF)

5 µg/mL

免疫沉淀 (IP)

1-5 µg

其他PubMed (MISC)

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产品规格

种属反应

Bovine, Human, Mouse, Non-human primate, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

B4F8

抗原

Full length recombinant human GAP.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2175873

产品详细信息

MA4-001 detects GTPase activating protein (GAP) from human, mouse, rat, bovine and non-human primate tissues.

MA4-001 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. Immunofluorescence staining of GAP in mouse fibroblast cells with MA4-001 results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. MA4-001 immunoprecipitates GAP that is complexed with the GAP related p190.

The MA4-001 antigen is full length recombinant human GAP. Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 & SH3.

Reconstitute with PBS.

靶标信息

TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 4,8, and 10. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Differential expression of cytoskeletal regulatory factors in the adolescent prefrontal cortex: Implications for cortical development.

Journal of neuroscience research

Shapiro LP,Parsons RG,Koleske AJ,Gourley SL

MA4001 was used in western blot to investigate mechanisms by which synapses are refined, pruned, and stabilized in the prefrontal cortex during adolescence

Mon May 01 00:00:00 UTC 2017

Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus.

Cell

Settleman J,Narasimhan V,Foster LC,Weinberg RA

MA4-001 was used in immunoprecipitation and western blot to clone the GAP-associated protein p190 and to investigate its functional properties

Fri May 01 00:00:00 UTC 1992

生物信息学

蛋白别名: GAP; GTPase-activating protein; p120 RAS GTPase activating protein; p120-rasGAP; p120GAP; Ras GTPase-activating protein 1; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein RAS p21); RAS p21 protein activator (GTPase activating protein) 1; RAS p21 protein activator 1; rasGAP; triphosphatase-activating protein

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基因别名: BOS_8464; CM-AVM; CMAVM; GAP; GAPX; p120; p120GAP; p120RASGAP; PKWS; RASA; RASA1; RASGAP

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UniProt ID: (Human) P20936, (Bovine) P09851, (Rat) P50904

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Entrez Gene ID: (Human) 5921, (Bovine) 282032, (Rat) 25676, (Mouse) 218397

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功能