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RASA3 Antibody (PA5-30445) in ICC/IF
RASA3 Polyclonal Antibody detects RASA3 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RASA3 protein stained by RASA3 Polyclonal Antibody (Product # PA5-30445) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-30445
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 583 and 834 of Human RASA3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547919
产品详细信息
Recommended positive controls: SK-N-SH, IMR32, SK-N-AS, mouse brain.
Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (86%), Chicken (89%), Bovine (92%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Human GAP1-InsP4 BP, also designated Ras p21 protein activator (GTPase-activating protein) 3 [Ins(1,3,4,5)P4-binding protein], is an 829-amino acid protein that binds phospholipids in both a calcium-dependent and -independent manner. GAP1, one of the Ras GTPase-activating protein families, comprises four distinct genes, including GAP1(m), GAP1-InsP4 BP, MRASAL (murine Ras GTPase-activating-like) and KIAA0538. This family contains an N-terminal tandem C2 domain, a GAP-related domain and a C-terminal pleckstrin homology (PH) domain. The PH domains of GAP1-InsP4 BP are essential for membrane targeting via binding of specific phospholipids. Following agonist-stimulated PtdIns(3,4,5)P(3) production, group I family PH domain containing proteins like GAP1-InsP4 BP specifically bind inositol phosphates, which are subsequently targeted to the plasma membrane.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GAP1(IP4BP); GAP1-InsP4 BP; GapIII; GAPIII activator 3; GTPase activating protein III; GTPase-activating protein 1 family, inositol 1,3,4,5-tetrakisphosphate-binding protein; Ins P4-binding protein; Ins(1,3,4,5)P4-binding protein; R-Ras gap; R-ras RTPase activating protein-3; Ras GTPase-activating protein 3; Ras GTPase-activating protein III; RP11-245B11.3
基因别名: AI326412; C86362; E130011G04; GAP1IP4BP; GAPIII; RASA3; scat
UniProt ID: (Human) Q14644, (Mouse) Q60790
Entrez Gene ID: (Human) 22821, (Mouse) 19414
MAPK cascade
signal transduction
positive regulation of GTPase activity
negative regulation of Ras protein signal transduction
release of sequestered calcium ion into cytosol
cellular response to heat
intracellular signal transduction
regulation of GTPase activity
calcium ion transmembrane transport
