Invitrogen

RASEF Recombinant Rabbit Monoclonal Antibody (JE65-33)

查看其他10个RASEF抗体

抗体检测数据 (4)

RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RASEF Antibody (MA5-44711) in IHC (P)

Immunohistochemistry analysis of RAB45 in paraffin-embedded rat large intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:100 for 1 hour at ro... View More

RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RASEF Antibody in Western Blot (WB)

RASEF Antibody in Flow Cytometry (Flow)

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RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB45 in paraffin-embedded rat large intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:100 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

RASEF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB45 in paraffin-embedded human thyroid tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:100 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

RASEF Antibody in Western Blot (WB) — Western blot analysis of RAB45 in different lysates (10 µg/Lane). Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Lane 1: K562 cell lysate; Lane 2: A431 cell lysate; Lane 3: RAW264.7 cell lysate; Lane 4: NIH/3T3 cell lysate. Predicted band size: 83 kDa. Observed band size: 85 kDa.

RASEF Antibody in Flow Cytometry (Flow) — Flow cytometry of RAB45 in HeLa cells. Cells were fixed and permeabilized then stained with RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1 µg/mL (red) at 4&(8)451; for an hour followed by iFluor™ 488 conjugate-Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4&(8)451;. Rabbit IgG Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

产品信息

MA5-44711

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

-

免疫组化（石蜡） (IHC (P))

1:100

-

流式细胞分析 (Flow)

1:500-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE65-33

抗原

Synthetic peptide within human RAB45 aa 81-130/740.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2931169

靶标信息

Binds predominantly GDP, and also GTP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Ras and EF-hand domain-containing protein; Ras and EF-hand domain-containing protein homolog; Ras-related protein Rab-45

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基因别名: BC030882; RAB45; RASEF

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UniProt ID: (Human) Q8IZ41, (Mouse) Q5RI75

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Entrez Gene ID: (Human) 158158, (Rat) 298138, (Mouse) 242505

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calcium ion binding GTP binding GTPase activity GDP binding nucleotide binding

small GTPase mediated signal transduction intracellular protein transport vesicle docking involved in exocytosis metabolic process protein secretion regulation of exocytosis Rab protein signal transduction protein transport