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RCC1 Antibody (MA5-49237) in ICC/IF
Immunocytochemistry analysis of RCC1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in RCC1 Monoclonal antibody (Product # MA5-49237) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
MA5-49237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6B11E7
Immunogen
E.coli-derived human RCC1 recombinant protein (Position: A14-S421).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074895
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human A431 whole cell, human HepG2 whole cell, human Raji whole cell. IHC: human thyroiditis tissue, human colorectal adenocarcinoma tissue, human breast cancer tissue, human spleen tissue, human squamous cell lung carcinoma tissue. ICC/IF: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle regulatory protein; Chromosome condensation protein 1; guanine nucleotide-releasing protein; Regulator of chromosome condensation; SNHG3-RCC1 readthrough
Gene Aliases: CHC1; RCC1; RCC1-I; SNHG3-RCC1
UniProt ID: (Human) P18754
Entrez Gene ID: (Human) 1104
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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