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FIGURE: 1 / 9
RCN2 Antibody (PA5-100076) in ICC/IF
Immunofluorescent analysis of RCN2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and RCN2 polyclonal antibody (Product # PA5-100076) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RCN2(Accession Q14257), corresponding to amino acid residues L277-L317.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815606
Product Specific Information
Antibody detects endogenous levels of total RCN2.
Target Information
The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium-binding protein ERC-55; E6-binding protein; E6BP; reticulocalbin 2, EF-hand calcium binding domain (endoplasmic reticulum calcium-binding protein, 55kD); Reticulocalbin-2; taipoxin-associated calcium binding protein 49; Taipoxin-associated calcium-binding protein 49; TCBP-49
Gene Aliases: AA408742; E6BP; ERC-55; ERC55; RCN2; TCBP49
UniProt ID: (Human) Q14257, (Mouse) Q8BP92, (Rat) Q62703
Entrez Gene ID: (Human) 5955, (Mouse) 26611, (Rat) 29218
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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