Invitrogen

REA Polyclonal Antibody

REA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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REA Antibody (PA5-14133) in IHC (P)

Immunohistochemistry analysis of REA in paraffin-embedded human brain tissue. Samples were incubated with REA polyclonal antibody (Product # PA5-14133) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.... View More

REA Antibody in Immunomicroscopy, Proximity Ligation Assay (PLA) (IM, PLA)

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产品信息

PA5-14133

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:250

免疫细胞化学 (ICC/IF)

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in situ PLA (PLA)

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免疫电镜技术 (IM)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2164780

产品详细信息

This antibody is predicted to react with bovine and rat based on sequence homology.

靶标信息

PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. This protein functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. It is probably involved in regulating mitochondrial respiration activity and in aging.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes.

Nature immunology

Zhou H,Wang H,Yu M,Schugar RC,Qian W,Tang F,Liu W,Yang H,McDowell RE,Zhao J,Gao J,Dongre A,Carman JA,Yin M,Drazba JA,Dent R,Hine C,Chen YR,Smith JD,Fox PL,Brown JM,Li X

PA5-14133 was used in Western Blotting to study IRAK2 Myddosome functions as a critical link between inflammation and metabolism, representing a novel therapeutic target for patients with obesity.

Thu Oct 01 00:00:00 UTC 2020

Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical Properties.

Molecular cancer therapeutics

Cao L,Weetall M,Trotta C,Cintron K,Ma J,Kim MJ,Furia B,Romfo C,Graci JD,Li W,Du J,Sheedy J,Hedrick J,Risher N,Yeh S,Qi H,Arasu T,Hwang S,Lennox W,Kong R,Petruska J,Moon YC,Babiak J,Davis TW,Jacobson A,Almstead NG,Branstrom A,Colacino JM,Peltz SW

PA5-14133 was used in Western Blotting to characterise the activity of PTC299 and study its role in the treatment of haematologic malignancies.

Tue Jan 01 00:00:00 UTC 2019

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.

Cell

Wei Y,Chiang WC,Sumpter R,Mishra P,Levine B

PA514133 was used in immunocytochemistry, proximity ligation assay, and western blot to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation

Thu Jan 12 00:00:00 UTC 2017

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.

Cell

Wei Y,Chiang WC,Sumpter R,Mishra P,Levine B

Thu Jan 12 00:00:00 UTC 2017

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.

Cell

Wei Y,Chiang WC,Sumpter R,Mishra P,Levine B

Thu Jan 12 00:00:00 UTC 2017

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.

Cell

Wei Y,Chiang WC,Sumpter R,Mishra P,Levine B

Thu Jan 12 00:00:00 UTC 2017

Stabilin-1 expression defines a subset of macrophages that mediate tissue homeostasis and prevent fibrosis in chronic liver injury.

Proceedings of the National Academy of Sciences of the United States of America

Rantakari P,Patten DA,Valtonen J,Karikoski M,Gerke H,Dawes H,Laurila J,Ohlmeier S,Elima K,Hübscher SG,Weston CJ,Jalkanen S,Adams DH,Salmi M,Shetty S

Published figure using REA polyclonal antibody (Product # PA5-14133) in Western Blot

Tue Aug 16 00:00:00 UTC 2016

生物信息学

蛋白别名: B-cell associated protein; B-cell receptor associated protein 37; B-cell receptor-associated protein 37; B-cell receptor-associated protein BAP37; BAP-37; D-prohibitin; MGC117268; PHB2; phb2 {ECO:0000250|UniProtKB:Q99623}; Prohibitin-2; Repressor of estrogen receptor activity

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基因别名: AU044498; BAP; Bap-37; Bap37; Bcap27; BCAP37; hBAP; p22; PHB2; PNAS-141; REA

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UniProt ID: (Human) Q99623, (Mouse) O35129, (Rat) Q5XIH7

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Entrez Gene ID: (Human) 11331, (Mouse) 12034, (Rat) 114766

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功能

protein binding protein C-terminus binding estrogen receptor binding amide binding protein N-terminus binding chromatin binding molecular_function

参与通路