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RFC3 Antibody (PA5-103161) in ICC/IF
Immunocytochemistry analysis of RFC3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RFC3 polyclonal antibody (Product # PA5-103161) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103161
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RFC3(Accession P40938), corresponding to amino acid residues M1-G42.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852531
产品详细信息
Antibody detects endogenous levels of total RFC3.
靶标信息
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A1 38 kDa subunit; Activator 1 38 kDa subunit; Activator 1 subunit 3; DNA-binding; MGC5276; Polymorphism; replication factor C (activator 1) 3, 38kDa; replication factor C 3; Replication factor C 38 kDa subunit; Replication factor C subunit 3; replication factor C, 38 kDa; RF-C 38 kDa subunit; RFC, 38 kD subunit; RFC38
基因别名: 2810416I22Rik; 38kDa; AU022547; Recc3; RFC3; RFC38
UniProt ID: (Human) P40938, (Mouse) Q8R323
Entrez Gene ID: (Human) 5983, (Rat) 288414, (Mouse) 69263
telomere maintenance via recombination
DNA synthesis involved in DNA repair
DNA replication
DNA strand elongation involved in DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
translesion synthesis
nucleotide-excision repair, DNA incision
error-prone translesion synthesis
DNA damage response, detection of DNA damage
response to organophosphorus
error-free translesion synthesis
positive regulation of DNA-directed DNA polymerase activity
regulation of signal transduction by p53 class mediator
DNA-dependent DNA replication
biological_process
