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RFP Antibody (600-901-379) in WB
Western Blot Results of Chicken Anti-RFP Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: RFP, load 50ng. Primary Antibody: Anti-RFP 1µg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Chicken HRP (p/n 603-103-126) at 1:40,000 for 30min at RT. Blocking: BlockOut (p/n MB-073) for 30min at RT. Expect: 27kDa.
产品信息
600-901-379
产品规格
种属反应
Tag
宿主/亚型
Chicken
/ IgY
分类
Polyclonal
类型
Antibody
抗原
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
selective precipitation
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot.
靶标信息
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP; RFP tag
