Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
RFP Antibody (600-906-379) in WB
Western Blot of Chicken Anti-RFP Antibody Biotin Conjugated. Lane 1: Super Signal Molecular Weight. Lane 2: 50ng of RFP. Primary Antibody: Chicken Anti-RFP biotin conjugated at 1:1000 overnight at 2-8°C. Secondary Antibody: HRP Streptavidin (p/n S000-03) 1:40,000 for 30mins at RT. Block: BlockOut Universal Blocking buffer (p/n MB-073). Expect: 27kDa.
产品信息
600-906-379
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Chicken
/ IgY
分类
Polyclonal
类型
Antibody
抗原
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
selective precipitation
保存液
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.
靶标信息
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP; RFP tag
