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RFP Antibody (PA1-986) in ICC/IF
Immunofluorescent analysis of recombinant Red Fluorescent Protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid (Product # 82026), and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with an anti-RFP polyclonal antibody (Product # PA1-986) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with... View More
产品信息
PA1-986
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_560939
产品详细信息
PA1-986 detects RFP tagged proteins. This antibody is specific for the RFP tag and does not detect GFP or GFP tagged proteins.
PA1-986 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of RFP tagged proteins. PA1-986 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples.
PA1-986 immunogen is a synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag. This peptide (Cat. # PEP-260) is available for use in neutralization and control experiments.
靶标信息
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP; RFP tag
