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RFP Antibody (200-301-379) in WB
Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
产品信息
200-301-379
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
8E5.G7
抗原
Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
偶联物
形式
Liquid
浓度
1.02 mg/mL
纯化类型
Protein A
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
靶标信息
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP; RFP tag
