Invitrogen

RGR Polyclonal Antibody

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RGR Antibody in Western Blot (WB)

RGR Antibody in Western Blot (WB)

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RGR Antibody (PA5-100993) in WB

Western blot analysis of RGR in ACHN cell lysate. Samples were incubated with RGR polyclonal antibody (Product # PA5-100993).

RGR Antibody in Western Blot (WB)

RGR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RGR Antibody in Immunohistochemistry (IHC)

RGR Antibody in Western Blot (WB)

RGR Antibody in Western Blot (WB)

RGR Antibody in Western Blot (WB)

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RGR Antibody in Western Blot (WB) — Western blot analysis of RGR in ACHN cell lysate. Samples were incubated with RGR polyclonal antibody (Product # PA5-100993).

RGR Antibody in Western Blot, Immunohistochemistry (WB, IHC) — FIGURE 3 UVA and UVR may not induce RGR expression in human keratinocytes. (A) The differential RGR expression in the exposed and unexposed skin tissues was analyzed by immunohistochemistry. The RGR was highly expressed in the epithelial layer of light-exposed sites, particularly in the basal cell layer and spinous cell layer of the epidermis and cells of the skin appendages. Exposed area vs unexposed area, * p < 0.05. Scale bar = 100 mum (right), 50 mum (middle), 20 mum (left). (B) Keratinocytes with all-trans-retinal were irradiated with 3 J/cm 2 UVA. Representative expression of RGR and beta -tubulin (loading control) by Western blot analysis. The relative protein levels of RGR and beta -tubulin were measured using ImageJ software (n = 3 independent experiments). (C) Keratinocytes with all-trans-retinal were irradiated with 1.5 J/cm 2 UVR. Representative expression of RGR and beta -tubulin (loading control) was detected by Western blot analysis. The relative protein levels of RGR and beta -tubulin were measured using ImageJ software (n = 3 independent experiments).

RGR Antibody in Immunohistochemistry (IHC) — FIGURE 1 RGR expression in human skin tissue. Immunohistochemical methods were used to show positive RGR staining in the epithelial layer (yellow arrow), sebaceous gland (black arrow), hair follicle (orange arrow), sweat gland (red arrow), and blood vessel (blue arrow) of skin tissue. Scale bar = 500 mum.

RGR Antibody in Western Blot (WB) — FIGURE 5 RGR regulates the proliferation, migration, and apoptosis of keratinocytes. (A) Representative keratinocyte defects in RGR (with small siRNA) and beta -tubulin (loading control) were detected by WB analysis. The relative protein levels of RGR and beta -tubulin were measured using ImageJ software (n = 3 independent experiments). * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Representative images of cell fragmentation and cell contraction observed under a light microscope in the si-RGR transfection group. No significant morphological change was detected in the si-control group. Scale bar = 20 mum * p < 0.05, ** p < 0.01, *** p < 0.001 vs the control group. (C) Flow cytometry with annexin V FITC/PI double staining was used to determine apoptotic cells. Following RGR knockdown, a significant decrease in apoptosis was detected in the keratinocytes transfected with si-RGR compared with the control. * p < 0.05, ** p < 0.01, *** p < 0.001 vs the control group. (D) Flow cytometry was used to detect the cell cycle after RGR knockdown in keratinocytes. Following RGR knockdown, a significant decrease in the S phase at 48 h was detected in keratinocytes transfected with si-RGR compared with the control. * p < 0.05 vs the control group. (E) RGR in keratinocytes was inhibited via Si-RGR, and cell viability was measured after 48 h. (F) Representative images from wound healing experiments showed low expression of the RGR and decreased mobility relative to controls. Scale bar = 50 mum *

RGR Antibody in Western Blot (WB) — FIGURE 2 RGR expression level and subcellular localization in human skin cells. (A) In normal human skin tissue, coimmunostaining of RGR (red) with the melanocyte marker melan-A (green) and keratinocyte marker P-CK (pink) is shown. Scale bar = 20 mum. The results showed positive staining of the RGR in the basal layer of the skin, large sudoriferous glands, hair follicles, and eccrine glands. Scale bar = 200 mum. (B) Representative expression of RGR and beta -tubulin (loading control) in melanocytes, keratinocytes, and fibroblasts, as measured by the Western blot assay (anti-RGR antibody: a band of ~32 kDa; beta -tubulin antibody: a band of ~55 kDa). The relative protein levels of RGR and beta -tubulin were measured by ImageJ software (n = 3 independent experiments). (C) In cultured cells, the colocalization of the RGR (green) and melan-A (red) in melanocytes, colocalization of RGR (green) and CK-pan (red) in keratinocytes, and colocalization of RGR (green) and vimentin (red) in fibroblasts were detected by immunofluorescence double staining. Scale bar = 20 mum. (D) Localization and expression of the RGR (green) in human skin cells (melanocytes, keratinocytes, and fibroblasts) were observed by a confocal microscope. Scale bar = 20 mum. (E) IEM staining of the normal skin tissue. Representative protein A-gold probe represents RGR (RGR is mainly located in the nucleoplasm (yellow arrow) and mitochondria (red arrow) and scattered in the cytoplasm and other organelles (black arrow

RGR Antibody in Western Blot (WB) — FIGURE 4 RGR is highly expressed in proliferative diseases and tumor tissues. (A) RGR expression (red) is colocalized with the proliferative marker PCNA (green) in psoriasis, seborrheic keratosis, and squamous cell carcinoma. Scale bar = 20 mum. (B) Representative expression of RGR and beta -tubulin (loading control) in lesion areas and non-lesion areas was detected by WB analysis. The relative protein levels of RGR and beta -tubulin were measured using ImageJ software (n = 3 independent experiments). * p < 0.05. (C) Representative expression of PCNA and beta -tubulin (loading control) was detected by WB analysis. The relative protein levels of RGR and beta -tubulin were measured using ImageJ software (n = 3 independent experiments). ** p < 0.01.

产品信息

PA5-100993

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫电镜技术 (IM)

-

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RGR(Accession P47804), corresponding to amino acid residues S130-M180.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2850474

产品详细信息

Antibody detects endogenous levels of total RGR.

靶标信息

This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes.

Frontiers in cell and developmental biology

Gu Y,Wang Y,Lan Y,Feng J,Zeng W,Zhang W,Lu H

Published figure using RGR polyclonal antibody (Product # PA5-100993) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes.

Frontiers in cell and developmental biology

Gu Y,Wang Y,Lan Y,Feng J,Zeng W,Zhang W,Lu H

Published figure using RGR polyclonal antibody (Product # PA5-100993) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes.

Frontiers in cell and developmental biology

Gu Y,Wang Y,Lan Y,Feng J,Zeng W,Zhang W,Lu H

Published figure using RGR polyclonal antibody (Product # PA5-100993) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes.

Frontiers in cell and developmental biology

Gu Y,Wang Y,Lan Y,Feng J,Zeng W,Zhang W,Lu H

Published figure using RGR polyclonal antibody (Product # PA5-100993) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes.

Frontiers in cell and developmental biology

Gu Y,Wang Y,Lan Y,Feng J,Zeng W,Zhang W,Lu H

Published figure using RGR polyclonal antibody (Product # PA5-100993) in Immunohistochemistry

Sat Jul 16 00:00:00 UTC 2022

生物信息学

蛋白别名: RGR-opsin; RPE retinal G-protein coupled receptor; RPE-retinal G protein-coupled receptor

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基因别名: RGR; RP44

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UniProt ID: (Human) P47804

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Entrez Gene ID: (Human) 5995

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G-protein coupled receptor activity protein binding photoreceptor activity G-protein coupled receptor

G-protein coupled receptor signaling pathway visual perception phototransduction protein-chromophore linkage