Invitrogen

RGS4 Polyclonal Antibody

RGS4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RGS4 Antibody (PA5-22332) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RGS4 (Product # PA5-22332) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

RGS4 Antibody — RGS4 controlled morphine-induced reward in the NAc (A) Schematic representation of the shRNA-targeting RGS4 construct. (B) Injection target site in the mouse brain (NAc; red circle). (C) Representative photographs of eGFP expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. (D) Representative immunoblots of RGS4 expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. All data are expressed as means ± SEM; n = 6 for each group. (E) Schematic diagram of the schedule for the morphine CPP test. (F) Mice infected with Lv-shRNA-eGFP in the NAc showed an increased response to morphine (15 mg/kg, i.p.) in the CPP paradigm. All data are expressed as means ± SEM (n = 6 for each group). Data were analyzed with unpaired t-tests. *p < 0.05 vs. the Lv-eGFP infected group. {KD}

产品信息

PA5-22332

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 205 of RGS4 (Uniprot ID#P49798)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.21 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155787

产品详细信息

Recommended positive controls: Raji, mouse cerebellum, rat brain.

Predicted reactivity: Mouse (95%), Rat (96%), Pig (95%), Rabbit (95%), Sheep (96%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。