Invitrogen

RHBDD1 Polyclonal Antibody

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RHBDD1 Antibody (PA5-20860) in WB

Western Blot analysis in A549 Cells. Loading: 15 µg of cell lysate. Antibodies: RHBDD1 Polyclonal Antibody (Product # PA5-20860) 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Exposure: 1 min.

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产品信息

PA5-20860

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 14 amino acid peptide from near the carboxy terminus of human RHBDD1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11157222

产品详细信息

A suggested positive control is K562 cell lysate.

PA5-20860 can be used with blocking peptide PEP-0974.

靶标信息

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。