Invitrogen

RHEB Polyclonal Antibody

RHEB Antibody in Immunocytochemistry (ICC/IF)

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RHEB Antibody (PA5-102373) in ICC/IF

Immunofluorescence analysis of RHEB was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RHEB Polyclonal Antibody (Product # PA5-102373) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

RHEB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RHEB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RHEB siRNA and loss of signal was observed in Western Blot application using Anti-RHEB Polyclonal Antibody (Product # PA5-102373). {KD}

产品信息

PA5-102373

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RHEB(Accession Q15382), corresponding to amino acid residues T42-S92.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2851780

产品详细信息

Antibody detects endogenous levels of total RHEB.

靶标信息

Rheb encodes a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

仅用于科研。不用于诊断过程。未经明确授权不得转售。