Invitrogen

RICTOR Monoclonal Antibody (7B3)

RICTOR Antibody in Immunocytochemistry (ICC/IF)

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RICTOR Antibody (MA5-15681) in ICC/IF

Immunofluorescent analysis of Rictor (magenta) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a Rictor monoclonal antibody (Product # MA5-15681) at a dilution of 1:200 in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-mouse IgG Secondary Antibody, Alexa Fluor 647 conjugate (Product # A32728) at a dilution of 1:1000 for 1 hou... View More

RICTOR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RICTOR Antibody in Flow Cytometry (Flow)

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产品信息

MA5-15681

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

7B3

抗原

Purified recombinant fragment of human RICTOR expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_10987411

产品详细信息

MA5-15681 targets RICTOR in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15681 immunogen is purified recombinant fragment of human RICTOR expressed in E. Coli.

MA5-15681 detects RICTOR which has a predicted molecular weight of approximately 192kDa.

靶标信息

RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

Cancers

Moritz S,Krause M,Schlatter J,Cordes N,Vehlow A

MA5-15681 was used in Proximity Ligation Assay (PLA) to show that the cytoskeleton regulator Lamellipodin mediates invasiveness, proliferation and radiosensitivity of glioblastoma cells.

Sun Oct 24 00:00:00 UTC 2021

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Nature communications

Mathieu J,Detraux D,Kuppers D,Wang Y,Cavanaugh C,Sidhu S,Levy S,Robitaille AM,Ferreccio A,Bottorff T,McAlister A,Somasundaram L,Artoni F,Battle S,Hawkins RD,Moon RT,Ware CB,Paddison PJ,Ruohola-Baker H

MA5-15681 was used in Western Blotting to demonstrate the mechanisms behind how cells exit human pluripotency by CRISPR-Cas9.

Thu Feb 07 00:00:00 UTC 2019

生物信息学

蛋白别名: AVO3 homolog; DKFZp686B11164; hAVO3; mAVO3; MGC39830; pianissimo; Protein pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3

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基因别名: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik; hAVO3; KIAA1999; PIA; RICTOR

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UniProt ID: (Human) Q6R327, (Mouse) Q0VAV4

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Entrez Gene ID: (Human) 253260, (Mouse) 78757

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功能

protein binding enzyme activator activity protein kinase binding ribosome binding

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