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RNF14 Antibody (MA5-27387) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a RNF14 monoclonal antibody (Product # MA5-27387) at a dilution of 1:500.
产品信息
MA5-27387
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
OTI1C9
抗原
Human recombinant protein fragment corresponding to amino acids 1-220 of RNF14 produced in E.coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 1% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2725249
靶标信息
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: androgen receptor associated protein 54; Androgen receptor-associated protein 54; E3 ubiquitin-protein ligase RNF14; HFB30; protein Triad2; RING finger protein 14; TRIAD2 type I
基因别名: 2310075C09Rik; 2610005D23Rik; AA986456; ARA54; AU041447; D18Ertd188e; D7Bwg0165e; HFB30; HRIHFB2038; RNF14; TRIAD2
UniProt ID: (Human) Q9UBS8, (Mouse) Q9JI90
Entrez Gene ID: (Human) 9604, (Mouse) 56736
transcription coactivator activity
protein binding
zinc ion binding
ligase activity
ubiquitin-like protein transferase activity
ubiquitin conjugating enzyme binding
androgen receptor binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein polyubiquitination
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
protein ubiquitination
androgen receptor signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of transcription, DNA-templated
regulation of androgen receptor signaling pathway
