Invitrogen

RNF146 Recombinant Rabbit Monoclonal Antibody (PSH0-29)

RNF146 Antibody in Immunocytochemistry (ICC/IF)

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RNF146 Antibody (MA5-50800) in ICC/IF

Immunocytochemistry analysis of RNF146 in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with RNF146 recombinant monoclonal antibody (Product # MA5-50800) with a dilution of 1:100 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of t... View More

RNF146 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RNF146 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-50800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH0-29

抗原

Recombinant protein within full length human RNF146 aa 1-359/359.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3093723

产品详细信息

Positive control: SH-SY5Y cell lysate, NIH/3T3 cell lysate, human testis tissue, human thyroid cancer tissue, NIH/3T3 cell, SH-SY5Y cell.

Predicted band size: 39 kDa

Subcellular Location: Cytoplasm, Nucleus.