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RNF185 Antibody (PA5-78615) in WB
RNF185 antibody detects RNF185 protein by western blot analysis. Non-transfected (-) and RNF185-transfected (+, ) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNF185 antibody RNF185 Polyclonal Antibody (Product # PA5-78615) at a dilution of 1:5,000.
产品信息
PA5-78615
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human RNF185. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.68 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736531
产品详细信息
Positive Control: human RNF185-transfected 293T
Predicted Reactivity: Mouse (98%), Rat (98%), Zebrafish (83%), Xenopus laevis (91%), Chicken (87%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
RNF185 is a E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. RNF185 acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. RNF185 protects cells from ER stress-induced apoptosis. is responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway, and is preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BSK65-MONO1; BSK65-MONO2; BSK65-PANC1; BSK65-PANC2; BSK65-TEST1; BSK65-TEST2; BSK65-TEST3; E3 ubiquitin-protein ligase RNF185; FLJ38628; RING finger protein 185; rn185
基因别名: RNF185
UniProt ID: (Human) Q96GF1
Entrez Gene ID: (Human) 91445
