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RNF31 Antibody (PA5-115849) in ICC/IF
Immunocytochemistry analysis of RNF31 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115849) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115849
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RNF31(Accession Q96EP0), corresponding to amino acid residues E814-L864.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900483
产品详细信息
Antibody detects endogenous levels of total RNF31/HOIP.
靶标信息
Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: E3 ubiquitin-protein ligase RNF31; FLJ23501; HOIL-1-interacting protein; HOIP; MGC19975; PAUL; Putative Ariadne-like ubiquitin ligase; RING finger protein 31; RING-type E3 ubiquitin transferase RNF31; Zinc in-between-RING-finger ubiquitin-associated domain protein
基因别名: AL033293; BC031509; Flj10111; HOIP; mFLJ00217; Paul; RNF31; ZIBRA
UniProt ID: (Human) Q96EP0, (Mouse) Q924T7
Entrez Gene ID: (Human) 55072, (Mouse) 268749, (Rat) 364386
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
ubiquitin binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
I-kappaB kinase/NF-kappaB signaling
regulation of tumor necrosis factor-mediated signaling pathway
CD40 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
protein linear polyubiquitination
positive regulation of protein targeting to mitochondrion
protein ubiquitination
negative regulation of necroptotic process
