Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), PerCP-eFluor™ 710, eBioscience™

ROR gamma (t) Antibody in Flow Cytometry (Flow)

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ROR gamma (t) Antibody (46-6981-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.125 µg of Anti-Mouse ROR gamma (t) PerCP-eFluor® 710 (right). Cells were also stained with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and Anti-Mouse CD19 eFluor® 450 (Product # 48-0193-82) and Anti-Mouse CD3 APC (Product # 17-0031-82). Viable, CD3. CD19. cells were used for analysis.

ROR gamma (t) Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

46-6981-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

B2D

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10717956

产品详细信息

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The ROR gamma t isoform differs from RORgamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.

Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Divergent metabolic programmes control two populations of MAIT cells that protect the lung.

Nature cell biology

Riffelmacher T,Paynich Murray M,Wientjens C,Chandra S,Cedillo-Castelán V,Chou TF,McArdle S,Dillingham C,Devereaux J,Nilsen A,Brunel S,Lewinsohn DM,Hasty J,Seumois G,Benedict CA,Vijayanand P,Kronenberg M

46-6981-82 was used in Flow cytometry/Cell sorting to investigate the metabolic dependencies that enable tuning of memory-like MAIT cell responses following pulmonary immunization with a Salmonella vaccine strain, revealing distinct CD127+ and Klrg1+ MAIT cell populations with divergent metabolic profiles and functions.

Thu Jun 01 00:00:00 UTC 2023

Multi-tissue single-cell analysis deconstructs the complex programs of mouse natural killer and type 1 innate lymphoid cells in tissues and circulation.

Immunity

McFarland AP,Yalin A,Wang SY,Cortez VS,Landsberger T,Sudan R,Peng V,Miller HL,Ricci B,David E,Faccio R,Amit I,Colonna M

46-6981-82 was used in Flow cytometry/Cell sorting to identify gene expression programs of tissue-specific ILC1s, tissue-specific NK cells, and non-tissue-specific populations in blood, spleen, and other tissues largely corresponding to circulating cells.

Tue Jun 08 00:00:00 UTC 2021

Vitamin D has an effect on airway inflammation and Th17/Treg balance in asthmatic mice.

The Kaohsiung journal of medical sciences

Ma JG,Wu GJ,Xiao HL,Xiao YM,Zha L

46-6981-82 was used in Flow cytometry/Cell sorting to explore the mechanism of vitamin D on asthma.

Wed Dec 01 00:00:00 UTC 2021

CD4+ effector T cells accelerate Alzheimer's disease in mice.

Journal of neuroinflammation

Machhi J,Yeapuri P,Lu Y,Foster E,Chikhale R,Herskovitz J,Namminga KL,Olson KE,Abdelmoaty MM,Gao J,Quadros RM,Kiyota T,Jingjing L,Kevadiya BD,Wang X,Liu Y,Poluektova LY,Gurumurthy CB,Mosley RL,Gendelman HE

46-6981-82 was used in Flow Cytometry to investigate the role of effector immunity and how it could affect cellular-level disease pathobiology in an alzheimer's disease animal model.

Fri Nov 19 00:00:00 UTC 2021

Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death.

Nature communications

Hayashi K,Nikolos F,Lee YC,Jain A,Tsouko E,Gao H,Kasabyan A,Leung HE,Osipov A,Jung SY,Kurtova AV,Chan KS

46-6981 was used in Flow cytometry/Cell sorting to postulate that an intricate balance between immunostimulatory and inhibitory DAMPs could determine the outcome of drug-induced ICD and pose COX-2/prostaglandin E2 blockade as a strategy to harness ICD.

Mon Dec 07 00:00:00 UTC 2020

Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death.

Nature communications

Hayashi K,Nikolos F,Lee YC,Jain A,Tsouko E,Gao H,Kasabyan A,Leung HE,Osipov A,Jung SY,Kurtova AV,Chan KS

Mon Dec 07 00:00:00 UTC 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

46-6981 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 UTC 2020

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

Yang BH,Wang K,Wan S,Liang Y,Yuan X,Dong Y,Cho S,Xu W,Jepsen K,Feng GS,Lu LF,Xue HH,Fu W

46-6981-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

Tue Jun 18 00:00:00 UTC 2019

Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.

Nature communications

Cheng HW,Onder L,Novkovic M,Soneson C,Lütge M,Pikor N,Scandella E,Robinson MD,Miyazaki JI,Tersteegen A,Sorg U,Pfeffer K,Rülicke T,Hehlgans T,Ludewig B

46-6981 was used in Flow cytometry/Cell sorting to establish fibroblastic reticular cell (FRC)- specific fate-mapping in mice to identify the embryonic origin and differentiation trajectories in the splenic white pulp.

Mon Apr 15 00:00:00 UTC 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using ROR gamma (t) monoclonal antibody (Product # 46-6981-82) in Flow Cytometry

Thu Jan 25 00:00:00 UTC 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using ROR gamma (t) monoclonal antibody (Product # 46-6981-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

Interleukin-33-Activated Islet-Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production.

Immunity

Dalmas E,Lehmann FM,Dror E,Wueest S,Thienel C,Borsigova M,Stawiski M,Traunecker E,Lucchini FC,Dapito DH,Kallert SM,Guigas B,Pattou F,Kerr-Conte J,Maechler P,Girard JP,Konrad D,Wolfrum C,Böni-Schnetzler M,Finke D,Donath MY

46-6981 was used in Flow cytometry/Cell sorting to provide evidence that an immunometabolic crosstalk between islet-derived IL-33, ILC2s, and myeloid cells fosters insulin secretion.

Tue Nov 21 00:00:00 UTC 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

46-6981 was used in Flow cytometry/Cell sorting to highlight that intratumoral immunomodulation with an oncolytic virus expressing a rationally selected ligand can be an effective strategy to drive systemic efficacy of immune checkpoint blockade.

Mon Feb 13 00:00:00 UTC 2017

Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses.

Nature immunology

Halim TY,Hwang YY,Scanlon ST,Zaghouani H,Garbi N,Fallon PG,McKenzie AN

46-6981 was used in Flow cytometry/Cell sorting to investigate the role of ILC2 cells in allergic inflammation, showing that they license dendritic cells to potentiate memory TH2 cell responses.

Fri Jan 01 00:00:00 UTC 2016

Leptin receptor signaling in T cells is required for Th17 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Reis BS,Lee K,Fanok MH,Mascaraque C,Amoury M,Cohn LB,Rogoz A,Dallner OS,Moraes-Vieira PM,Domingos AI,Mucida D

Published figure using ROR gamma (t) monoclonal antibody (Product # 46-6981-82) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2015

Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.

Nature medicine

Kim HY,Lee HJ,Chang YJ,Pichavant M,Shore SA,Fitzgerald KA,Iwakura Y,Israel E,Bolger K,Faul J,DeKruyff RH,Umetsu DT

Published figure using ROR gamma (t) monoclonal antibody (Product # 46-6981-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

Nuclear orphan receptor NR2F6 directly antagonizes NFAT and RORγt binding to the Il17a promoter.

Journal of autoimmunity

Hermann-Kleiter N,Meisel M,Fresser F,Thuille N,Müller M,Roth L,Katopodis A,Baier G

Published figure using ROR gamma (t) monoclonal antibody (Product # 46-6981-82) in Flow Cytometry

Sat Dec 01 00:00:00 UTC 2012

RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells.

Nature immunology

Sanos SL,Bui VL,Mortha A,Oberle K,Heners C,Johner C,Diefenbach A

Thu Jan 01 00:00:00 UTC 2009

生物信息学

蛋白别名: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR

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基因别名: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR

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UniProt ID: (Mouse) P51450

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Entrez Gene ID: (Mouse) 19885

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功能