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RORA Antibody (PA5-11224) in ICC/IF
Immunocytochemistry analysis of RORA in HeLa cells. Samples were incubated with RORA polyclonal antibody (Product # PA5-11224) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). RORA im... View More
产品信息
PA5-11224
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 193-222 amino acids from human RORA
偶联物
形式
Liquid
浓度
2.0 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2285213
产品详细信息
This antibody is predicted to react with mouse based on sequence homology.
靶标信息
RORA is a receptor for retinoic acid belonging to the NR1 subfamily of nuclear hormone receptors with a nuclear receptor DNA binding domain. RORA binds either as a monomer or as a homodimer to the retinoic acid response element and thus regulates gene expression and also controls cell function. Though the exact function of RORA is yet unknown, it interacts with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, NM23-1, the product of a tumor metastasis suppressor candidate gene, CDK7, NCOA3 and NCOA6 coactivators. It is found to express in mesenchymal stem cells derived from bone marrow and are known to act by direct modulation of a bone matrix component. It is widely expressed in most of the tissues. Chromosomal aberrations involving RORA may be a cause of acute promyelocytic leukemia (APL).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR Alpha; ROR-alpha; Roralpha; thyroid hormone nuclear receptor alpha variant 4; transcription factor RZR-alpha
基因别名: NR1F1; ROR1; ROR2; ROR3; RORA; RZR-ALPHA; RZRA
UniProt ID: (Human) P35398
Entrez Gene ID: (Human) 6095
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcription corepressor binding
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
beta-catenin binding
transcription factor binding
oxysterol binding
zinc ion binding
sequence-specific DNA binding
transcription factor activity, direct ligand regulated sequence-specific DNA binding
C4 zinc finger nuclear receptor
angiogenesis
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
xenobiotic metabolic process
nitric oxide biosynthetic process
regulation of smoothened signaling pathway
positive regulation of vascular endothelial growth factor production
regulation of glucose metabolic process
regulation of steroid metabolic process
cerebellar Purkinje cell differentiation
cerebellar granule cell precursor proliferation
intracellular receptor signaling pathway
circadian regulation of gene expression
cellular response to sterol
muscle cell differentiation
positive regulation of circadian rhythm
regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
steroid hormone mediated signaling pathway
negative regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cGMP metabolic process
negative regulation of inflammatory response
regulation of transcription involved in cell fate commitment
triglyceride homeostasis
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to hypoxia
T-helper 17 cell differentiation
regulation of cholesterol homeostasis
