Invitrogen

RPA14 Polyclonal Antibody

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3 篇参考文献

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抗体检测数据 (2)
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RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RPA14 Antibody (PA5-21277) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer patient tumor, using RPA 14 kDa subunit (Product # PA5-21277) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPA14 Antibody in Western Blot (WB)

RPA14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPA14 Antibody in Western Blot (WB)

RPA14 Antibody in Western Blot (WB)

RPA14 Antibody in Western Blot (WB)

Product Image Gallery

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lung cancer patient tumor, using RPA 14 kDa subunit (Product # PA5-21277) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

RPA14 Antibody in Western Blot (WB) — Western Blot using RPA14 Polyclonal Antibody (Product # PA5-21277). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPA14 Polyclonal Antibody (Product # PA5-21277) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

RPA14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Western Blot and Immunocytochemistry

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Figure 5 High RPA 3 expression predicted a poor prognosis in patients with nasopharyngeal carcinoma ( NPC ). ( A ) Immunohistochemistry ( IHC ) assays for RPA 3 expression in 21 normal epithelium tissues and 104 NPC tissues. The left panel shows that RPA 3 expression was low in normal tissues. The middle panel shows low RPA 3 expression in NPC tissues. The right panel shows high RPA 3 expression in NPC tissues. The lower panels show magnified pictures of the boxed area in the corresponding upper panels. Scale bars, 50 mum. ( B ) RPA 3 expression level was compared between NPC and non-tumour epithelium tissues. ( C ) Overall survival ( OS ) of patients with NPC based on RPA 3 expression. ( D ) Recurrence-free survival ( RFS ) curve of patients with NPC based on RPA 3 expression. ( E ) Cumulative bar chart showing the correlation between RPA 3 expression and distant metastasis in patients with NPC .

RPA14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Immunohistochemistry (Paraffin)

RPA14 Antibody in Western Blot (WB) — Figure 2 RPA 3 regulates the radiosensitivity of nasopharyngeal carcinoma ( NPC ) cells. ( A ) Western blot showing the expression of RPA proteins in different NPC cell lines. ( B ) Western blot showing the effect of RPA 3 overexpression and RPA 3 knockdown in the indicated NPC cells. ( C ) Dose-survival curves of the indicated cells. ( D ) Proliferation curves of the indicated cells. No significant difference was observed. ( E ) The indicated cells were subjected to invasion assays as described. The representative photographs are shown in the left panel, and the right panel shows the quantification of invasion assays. No significant difference was observed. Scale bars, 200 mum.

RPA14 Antibody in Western Blot (WB) — Figure 4 Overexpression of RPA 3 enhanced RAD 51 foci formation after irradiation in NPC cells. ( A ) Representative confocal images showing cells immunostained with antibodies against RPA 3 and RAD 51 after irradiation. The indicated cells were irradiated (2 Gy) and immunostained 1 hr later with antibodies against RPA 3 and RAD 51; the nuclei were stained with DAPI . Scale bars, 5 mum. ( B ) Percentage of RAD 51 foci-positive cells. A positive cell was defined by the presence of more than 5 RAD 51 foci. * P < 0.05. ( C ) Western blot showing that the overexpression of RPA 3 did not affect RAD 51 expression.

RPA14 Antibody in Western Blot (WB) — Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Western Blot

产品信息

PA5-21277

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human RPA14 Recombinant protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11157424

产品详细信息

Recommended positive controls: 293T.

Predicted reactivity: Rhesus Monkey (94%), Bovine (86%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RPA3 is a potential marker of prognosis and radioresistance for nasopharyngeal carcinoma.

Journal of cellular and molecular medicine

Qu C,Zhao Y,Feng G,Chen C,Tao Y,Zhou S,Liu S,Chang H,Zeng M,Xia Y

Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Immunohistochemistry (Paraffin)

Wed Nov 01 00:00:00 UTC 2017

RPA3 is a potential marker of prognosis and radioresistance for nasopharyngeal carcinoma.

Journal of cellular and molecular medicine

Qu C,Zhao Y,Feng G,Chen C,Tao Y,Zhou S,Liu S,Chang H,Zeng M,Xia Y

Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Immunohistochemistry (Paraffin)

Wed Nov 01 00:00:00 UTC 2017

RPA3 is a potential marker of prognosis and radioresistance for nasopharyngeal carcinoma.

Journal of cellular and molecular medicine

Qu C,Zhao Y,Feng G,Chen C,Tao Y,Zhou S,Liu S,Chang H,Zeng M,Xia Y

Published figure using RPA14 polyclonal antibody (Product # PA5-21277) in Immunohistochemistry (Paraffin)

Wed Nov 01 00:00:00 UTC 2017

生物信息学

蛋白别名: Replication factor A protein 3; Replication protein A 14 kDa subunit; replication protein A3, 14kDa; RF-A protein 3; RP-A p14

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基因别名: REPA3; RP-A p14; RPA14; RPA3

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UniProt ID: (Human) P35244

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Entrez Gene ID: (Human) 6119

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damaged DNA binding single-stranded DNA binding protein binding

G1/S transition of mitotic cell cycle telomere maintenance via recombination double-strand break repair via homologous recombination DNA replication transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair regulation of mitotic cell cycle translesion synthesis nucleotide-excision repair, DNA incision interstrand cross-link repair regulation of cell proliferation error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis regulation of cellular response to heat regulation of signal transduction by p53 class mediator