Invitrogen

RPA2 Polyclonal Antibody

RPA2 Antibody in Immunocytochemistry (ICC/IF)

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RPA2 Antibody (PA5-22256) in ICC/IF

Immunofluorescence analysis of RPA2 was performed using 70% confluent log phase HeLa cells treated with 30 µmol/L Etoposide for 1 hour. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPA2 Polyclonal Antibody (product # PA5-22256) at 5µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Flu... View More

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RPA2 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of RPA2 using Anti-RPA2 Polyclonal Antibody (Product # PA5-22256) shows increased expression of proteins in HeLa cell line upon Etoposide treatment. {TM}

产品信息

PA5-22256

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 202 of RPA32 (Uniprot ID#P15927)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.64 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152792

产品详细信息

Recommended positive controls: 293T, A431, BCL-1, PC-12, Rat2.

Predicted reactivity: Mouse (90%), Rat (91%), Pig (93%), Chicken (81%), Rhesus Monkey (99%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E3 ligase RFWD3 is a novel modulator of stalled fork stability in BRCA2-deficient cells.

The Journal of cell biology

Duan H,Mansour S,Reed R,Gillis MK,Parent B,Liu B,Sztupinszki Z,Birkbak N,Szallasi Z,Elia AEH,Garber JE,Pathania S

PA5-22256 was used in Western Blotting to identify the role of E3 ligase RFWD3 in modulating stalled fork stability in BRCA2-deficient cells.

Mon Jun 01 00:00:00 UTC 2020

E3 ligase RFWD3 is a novel modulator of stalled fork stability in BRCA2-deficient cells.

The Journal of cell biology

Duan H,Mansour S,Reed R,Gillis MK,Parent B,Liu B,Sztupinszki Z,Birkbak N,Szallasi Z,Elia AEH,Garber JE,Pathania S

PA5-22256 was used in Western Blotting to identify the role of E3 ligase RFWD3 in modulating stalled fork stability in BRCA2-deficient cells.

Mon Jun 01 00:00:00 UTC 2020

Glutaminase and poly(ADP-ribose) polymerase inhibitors suppress pyrimidine synthesis and VHL-deficient renal cancers.

The Journal of clinical investigation

Okazaki A,Gameiro PA,Christodoulou D,Laviollette L,Schneider M,Chaves F,Stemmer-Rachamimov A,Yazinski SA,Lee R,Stephanopoulos G,Zou L,Iliopoulos O

PA5-22256 was used in Western Blotting to show that VHL-/- renal cell carcinoma cells rely on RC-deprived aspartate to maintain de novo pyrimidine biosynthesis.

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: 30-kDa protein; HSSB; p32; p32-subunit of replication protein A; p34; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RpLP1; RpP2

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基因别名: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

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UniProt ID: (Human) P15927, (Rat) Q63528

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Entrez Gene ID: (Human) 6118, (Mouse) 19891, (Rat) 59102

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功能

damaged DNA binding single-stranded DNA binding protein binding enzyme binding protein phosphatase binding ubiquitin protein ligase binding protein N-terminus binding DNA binding

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