Invitrogen

RPA2 Monoclonal Antibody (MA34)

RPA2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

RPA2 Antibody (MA1-870) in ICC/IF

Immunofluorescent analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody (Product # MA1-870) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RPA2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. RPA2 cells were transfected with RPA2 siRNA and reduction of signal was observed in Western Blot using RPA2 Monoclonal Antibody (MA34) (Product # MA1-870). {KD}

产品信息

MA1-870

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Non-human primate, Rat

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

MA34

抗原

RPA p34 subunit isolated from HeLa cells.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_795759

产品详细信息

MA1-870 detects RPA p34 in human and rat cells.

MA1-870 has been successfully used in Western blotting, immunofluorescence and immunohistochemistry (paraffin) procedures. By Western blot it detects a 34 kDa protein representing RPA p34.

The MA1-870 immunogen is the RPA p34 subunit isolated from HeLa cells.

靶标信息

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: HSSB; p32; p32-subunit of replication protein A; p34; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RpLP1; RpP2

查看更多收起

基因别名: REPA2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

查看更多收起

UniProt ID: (Human) P15927, (Rat) Q63528

查看更多收起

Entrez Gene ID: (Human) 6118, (Rat) 59102

查看更多收起

功能

damaged DNA binding single-stranded DNA binding protein binding enzyme binding protein phosphatase binding ubiquitin protein ligase binding protein N-terminus binding DNA binding

参与通路