Invitrogen

RPA70 Polyclonal Antibody

RPA70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

RPA70 Antibody (PA5-21976) in ICC/IF

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit antibody (Product # PA5-21976) at 1:200 dilution.

RPA70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RPA70 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RPA70 siRNA and decrease in signal was observed in Western Blot using RPA70 Polyclonal Antibody (Product # PA5-21976). {KD}

Product Details

PA5-21976

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (Uniprot ID#P27694)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_11154806

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.

Predicted reactivity: Mouse (87%), Rat (85%), Sheep (93%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Maternal Smc3 protects the integrity of the zygotic genome through DNA replication and mitosis.

Development (Cambridge, England)

Yueh WT,Singh VP,Gerton JL

PA5-21976 was used in Immunocytochemistry-immunofluorescence to suggest that, despite previous reports of aneuploidy in early embryos, chromosome missegregation in zygotes halts embryogenesis at the two-cell stage.

Wed Dec 15 00:00:00 UTC 2021

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

Published figure using RPA70 polyclonal antibody (Product # PA5-21976) in Immunofluorescence

Thu Aug 14 00:00:00 UTC 2014

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

Published figure using RPA70 polyclonal antibody (Product # PA5-21976) in Immunofluorescence

Thu Aug 14 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: MSTP075; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein

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Gene Aliases: 5031405K23Rik; 70kDa; AA589576; AW557552; HSSB; MST075; REPA1; RF-A; RP-A; Rpa; RPA1; RPA70

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UniProt ID: (Human) P27694, (Mouse) Q8VEE4

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Entrez Gene ID: (Human) 6117, (Mouse) 68275

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Function(s)

damaged DNA binding single-stranded DNA binding protein binding metal ion binding nucleic acid binding DNA binding chromatin binding

Process(es)

G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance double-strand break repair via homologous recombination DNA replication DNA-dependent DNA replication DNA repair transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA recombination protein sumoylation translesion synthesis nucleotide-excision repair, DNA incision interstrand cross-link repair error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis regulation of cellular response to heat regulation of signal transduction by p53 class mediator cell cycle checkpoint in utero embryonic development cellular response to DNA damage stimulus meiotic nuclear division positive regulation of cell proliferation hemopoiesis homeostasis of number of cells within a tissue chromosome organization

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RPA70 Polyclonal Antibody

RPA70 Antibody (PA5-21976) in ICC/IF

Product Details

PA5-21976

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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