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RPRD1B Antibody (PA5-148238) in ICC/IF
Immunocytochemistry analysis of RPRD1B in Hela cells. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 at 4° overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.
产品信息
PA5-148238
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human RPRD1B Protein (Met1-Asp326)
偶联物
形式
Liquid
浓度
100 µg/mL
纯化类型
Antigen affinity chromatography, Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; Regulation of nuclear pre-mRNA domain-containing protein 1B
基因别名: C20orf77; CREPT; dJ1057B20.2; NET60; RPRD1B
UniProt ID: (Human) Q9NQG5
Entrez Gene ID: (Human) 58490
