Invitrogen

RSK1 Polyclonal Antibody

RSK1 Antibody in Immunocytochemistry (ICC/IF)

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RSK1 Antibody (PA5-29215) in ICC/IF

Immunofluorescence analysis of RSK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RSK1 Rabbit Polyclonal Antibody (Product # PA5-29215) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

RSK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RSK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RSK1 siRNA and reduction of signal was observed in Western Blot using RSK1 Polyclonal Antibody (Product # PA5-29215). {KD}

产品信息

PA5-29215

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 47 and 276 of Human RSK1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546691

产品详细信息

Recommended positive controls: A431, HeLa, HepG2, Raji.

Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (97%), Pig (99%), Chicken (98%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

International journal of molecular sciences

Sakowicz A,Bralewska M,Pietrucha T,Figueras F,Habrowska-Górczyńska DE,Piastowska-Ciesielska AW,Gach A,Sakowicz B,Rybak-Krzyszkowska M,Huras H,Grzesiak M,Biesiada L

Published figure using RSK1 polyclonal antibody (Product # PA5-29215) in Immunocytochemistry

Wed Sep 22 00:00:00 UTC 2021

生物信息学

蛋白别名: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; p90-RSK 1; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6K-alpha 1; S6K-alpha-1

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基因别名: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1

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UniProt ID: (Human) Q15418, (Mouse) P18653

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Entrez Gene ID: (Human) 6195, (Mouse) 20111

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