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RTEL1 Antibody (PA5-79939) in WB
Western blot analysis of RTEL1 in Lane 1: rat testis tissue lysate, Lane 2: rat PC-12 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with RTEL1 polyclonal antibody (Product # PA5-79939) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by g... View More
产品信息
PA5-79939
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human RTEL1 recombinant protein (Position: R651-M756).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2747054
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DEAH helicase; helicase-like protein NHL; Novel helicase-like; Regulator of telomere elongation helicase 1; regulator of telomere elongation helicase 1 {ECO:0000255|HAMAP-Rule:MF_03065}; regulator of telomere length; RTEL1
基因别名: AI451565; AW540478; C20orf41; DKCA4; DKCB5; KIAA1088; NHL; PFBMFT3; RGD1306721; RTEL; RTEL1
UniProt ID: (Human) Q9NZ71, (Rat) Q5RJZ1, (Mouse) Q6H1L0
Entrez Gene ID: (Human) 51750, (Rat) 362288, (Mouse) 269400
DNA binding
ATP-dependent DNA helicase activity
protein binding
ATP binding
metal ion binding
4 iron, 4 sulfur cluster binding
nucleotide binding
nucleic acid binding
helicase activity
ATP-dependent helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
iron-sulfur cluster binding
DNA helicase
telomere maintenance
strand displacement
DNA repair
regulation of double-strand break repair via homologous recombination
replication fork processing
positive regulation of telomere maintenance
DNA duplex unwinding
telomere maintenance in response to DNA damage
telomeric loop disassembly
mitotic telomere maintenance via semi-conservative replication
positive regulation of telomere capping
positive regulation of telomere maintenance via telomere lengthening
negative regulation of t-circle formation
negative regulation of telomere maintenance in response to DNA damage
positive regulation of telomeric loop disassembly
nucleobase-containing compound metabolic process
cellular response to DNA damage stimulus
