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RTN3 Antibody (MA5-50827) in ICC/IF
Immunocytochemistry analysis of RTN3 in PC-12 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with RTN3 recombinant monoclonal antibody (Product # MA5-50827) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelle... View More
产品信息
MA5-50827
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH0-40
抗原
Recombinant protein within human RTN3 aa 1-500/1032.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3093728
产品详细信息
Positive control: HeLa cell lysate, HEK-293 cell lysate, U87-MG cell lysate, A549 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, L6 cell lysate, human cerebellum tissue lysate, PC-12, HeLa.
Predicted band size: 112.6 kDa
Subcellular Location: Endoplasmic reticulum membrane, Golgi apparatus membrane.
靶标信息
RTN3 is a member of reticulons, which are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. Alternatively spliced transcript variants encoding different isoforms have been identified. Multiple promoters rather than alternative splicing of internal exons seem to be involved in this diversity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ASY interacting protein; HAP; Homolog of ASY protein; Neuroendocrine-specific protein-like 2; Neuroendocrine-specific protein-like II; nsp-c; NSP-like protein 2; NSP-like protein II; Reticulon-3
基因别名: ASYIP; HAP; NSPL2; NSPLII; RTN3; RTN3-A1
UniProt ID: (Human) O95197, (Rat) Q6RJR6
Entrez Gene ID: (Human) 10313, (Rat) 140945
