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RUNX1 Antibody (701202) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of K562 (Lane 1), PC-3 (Lane 2) and Mouse Small Intestine (Lane 3). The blots were probed with Recombinant Rabbit Monoclonal Anti-RUNX1 Antibody (Product # 701202, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Recombinant Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 60 kDa corresponding to RUNX1 was observed in K562 and PC3, while ~ 48 kDa band was observed in mouse small in... View More
产品信息
701202
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
3H2L6
抗原
Peptide corresponding to amino acids 174-188 of human Runx1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532428
产品详细信息
This antibody is predicted to react with bovine and non-human primate based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor
基因别名: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1
UniProt ID: (Human) Q01196
Entrez Gene ID: (Human) 861, (Mouse) 12394
regulatory region DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
ATP binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
calcium ion binding
transcription factor binding
protein homodimerization activity
protein heterodimerization activity
repressing transcription factor binding
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
chondrocyte differentiation
transcription from RNA polymerase II promoter
hemopoiesis
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
hematopoietic stem cell proliferation
skeletal system development
in utero embryonic development
liver development
myeloid progenitor cell differentiation
regulation of T cell anergy
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
central nervous system development
negative regulation of cell proliferation
regulation of signal transduction
myeloid cell differentiation
neuron differentiation
negative regulation of granulocyte differentiation
hair follicle morphogenesis
response to retinoic acid
positive regulation of interferon-gamma production
embryonic hemopoiesis
negative regulation of transcription, DNA-templated
behavioral response to pain
neuron development
definitive hemopoiesis
regulation of hair follicle cell proliferation
cellular response to transforming growth factor beta stimulus
positive regulation of cell maturation
positive regulation of progesterone secretion
