Invitrogen

RUNX1 Monoclonal Antibody (RXDMC), PE, eBioscience™

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RUNX1 Antibody (12-9816-80) in Flow

C57Bl/6 thymocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82), followed by intracellular staining with 0.5 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.5 µg of Anti-Human/Mouse Runx1 PE using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). The histogram demonstrates Runx1 staining of CD4 SP (purple histogram) and CD8 SP (orange histogram) thymocytes.

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产品信息

12-9816-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RXDMC

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11151519

产品详细信息

Description: This RXDMC monoclonal antibody reacts with human and mouse Runx1, which is also known as AML1. This 50-kDa transcription factor is expressed in hematopoietic cells such as myeloid, T, and B cells, but not erythroid cells. Furthermore, Runx1 can be detected in nearly all non-hematopoietic tissues except the brain and heart. Runx1 is involved in several stages of T cell development, including the CD4-CD8- double negative (DN) to CD4+CD8+ double positive (DP) transition and commitment to the CD4 lineage. In addition to its role in thymocyte development, Runx1 has also been implicated in Th2 differentiation and immune homeostasis via direct interaction with Foxp3 in CD4+CD25+ regulatory T cells. Runx1 also affects Th2 differentiation by negatively regulating Gata-3 expression. Finally, the AML1 gene, which encodes Runx1, is a frequent target of translocations in acute myeloid leukemia.

In humans, alternative splicing results in the generation of eleven isoforms. In mice, five isoforms exist as a result of alternative splicing; isoform 4 (aka AML1-C) is the most highly expressed in hematopoietic cells. Sequence analysis indicates that the RXDMC antibody recognizes isoform 4 in mouse and all human isoforms except AML-1FA, AML-1FB, and AML-1FC.

Applications Reported: This RXDMC antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This RXDMC antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy.

Cell metabolism

Ye L,Park JJ,Peng L,Yang Q,Chow RD,Dong MB,Lam SZ,Guo J,Tang E,Zhang Y,Wang G,Dai X,Du Y,Kim HR,Cao H,Errami Y,Clark P,Bersenev A,Montgomery RR,Chen S

12-9816-80 was used in Flow cytometry/Cell sorting to provide a system for identification of GOF immune boosters and demonstrate PRODH2 as a target to enhance CAR-T efficacy.

Tue Apr 05 00:00:00 UTC 2022

A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development.

Nature communications

Mao AP,Ishizuka IE,Kasal DN,Mandal M,Bendelac A

Mon Oct 16 00:00:00 UTC 2017

生物信息学

蛋白别名: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor

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基因别名: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1

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UniProt ID: (Human) Q01196

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Entrez Gene ID: (Human) 861, (Mouse) 12394

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