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产品信息
31553
应用
建议稀释比
免疫组化 (IHC)
1:50 - 1:200
免疫细胞化学 (ICC/IF)
1:50 - 1:200
流式细胞分析 (Flow)
1:50 - 1:200
免疫沉淀 (IP)
1:50 - 1:200
产品规格
种属反应
Goat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Lyophilized
浓度
1.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient
RRID
AB_228400
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Antibody Specificity: This antibody reacts with the light chains on goat IgG and with those common to other goat immunoglobulins. The antibody does not react with the Fc portion of goat IgG. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)
Amax = 492 nm; Emax = 520 nm
Fluorophore/Protein: 9.1 µg/mg; 3.4 moles FITC per mole IgG
Restoration and Storage: Store product at 4°C until opened. After opening, restore to 1.1 mL with distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product is stable for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
