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Ras (G12V Mutant) Antibody (PA5-78086) in WB
Western Blot analysis of Ras (G12V Mutant) was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ras (G12V Mutant) Polyclonal Antibody (Product # PA5-78086) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
产品信息
PA5-78086
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RAS (mutant G12V). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.02 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736500
产品详细信息
Positive Control: NRAS(G12V)-transfected 293T
Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Chicken (100%), Bovine (100%), Guinea pig (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Ras is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The process of shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in Ras gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GTPase NRas; N-ras protein part 4; neuroblastoma RAS viral (v-ras) oncogene homolog; Transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
基因别名: ALPS4; CMNS; HRAS1; N-ras; NCMS; NRAS; NRAS1; NS6
UniProt ID: (Human) P01111
Entrez Gene ID: (Human) 4893
