Invitrogen

Recoverin Monoclonal Antibody (6a55 cd6)

Recoverin Antibody in Immunocytochemistry (ICC/IF)

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Recoverin Antibody (MA1-932) in ICC/IF

Immunofluorescent analysis of Recoverin (green) showing staining in the cytoplasm of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Recoverin monoclonal antibody (Product # MA1-932) in 3% BSA-PBS at ... View More

Recoverin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA1-932

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:500

免疫组织化学（多聚甲醛固定） (IHC (PFA))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:10-1:200

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Bovine, Human, Sheep, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

6a55 cd6

抗原

Proprietary Immunogen.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2178006

产品详细信息

MA1-932 will detect Recoverin from human, rat, bovine, and ovine samples.

MA1-932 has been successfully used in Western blot, ICC/IF and IHC (P) procedures. By Western blot, this antibody will detect a ~23 kDa band representing Recoverin from rat brain, as well as in bovine and ovine retina. This antibody is not recommended for rat brain tissue in IHC applications.

The MA1-932 immunogen is considered commercially sensitive.

靶标信息

Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.

Molecular therapy : the journal of the American Society of Gene Therapy

Tay HG,Andre H,Chrysostomou V,Adusumalli S,Guo J,Ren X,Tan WS,Tor JE,Moreno-Moral A,Plastino F,Bartuma H,Cai Z,Tun SBB,Barathi VA,Siew Wei GT,Grenci G,Chong LY,Holmgren A,Kvanta A,Crowston JG,Petretto E,Tryggvason K

MA1-932 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot to investigate the role of a human recombinant retina-specific laminin isoform, LN523 in promoting the differentiation of human embryonic stem cells into photoreceptor progenitors.

Wed Mar 01 00:00:00 UTC 2023

Compartmentalized microfluidic device for in vitro co-culture of retinal cells.

Biotechnology journal

Jahagirdar D,Yadav S,Gore M,Korpale V,Mathpati CS,Chidambaram S,Majumder A,Jain R,Dandekar P

MA1-932 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot to prove the functionality of the retinal coculture, which may be used as an appropriate perfusion-based, preclinical tool for the evaluation of novel retinal drugs and delivery systems.