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RhoG Antibody (PA5-99704) in ICC/IF
Immunofluorescent analysis of RhoG in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and RhoG polyclonal antibody (Product # PA5-99704) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-99704
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RHOG(Accession P84095), corresponding to amino acid residues Y64-G114.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818637
产品详细信息
Antibody detects endogenous levels of total Rho G.
靶标信息
The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: aplysia ras-related homolog G (RhoG); ras homolog G (RhoG); ras homolog gene family, member G (rho G); Rho G; Rho-related GTP-binding protein RhoG; Sid 10750
基因别名: 2810426G09Rik; ARHG; RHOG; Sid10750
UniProt ID: (Human) P84095, (Mouse) P84096
Entrez Gene ID: (Human) 391, (Mouse) 56212
Rho protein signal transduction
positive regulation of cell proliferation
Rac protein signal transduction
actin cytoskeleton organization
platelet activation
positive regulation of transcription, DNA-templated
regulation of small GTPase mediated signal transduction
cell chemotaxis
positive regulation of establishment of protein localization to plasma membrane
activation of GTPase activity
regulation of ruffle assembly
small GTPase mediated signal transduction
metabolic process
