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RhoGDI Antibody (PA5-27413) in ICC/IF
Immunofluorescence analysis of RhoGDI was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RhoGDI Polyclonal Antibody (Product # PA5-27413) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
产品信息
PA5-27413
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 204 of Human RhoGDI
偶联物
形式
Liquid
浓度
0.76 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2544889
产品详细信息
Recommended positive controls: NT2D, IMR32, Jurkat, HeLa, mouse brain.
Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (80%), Pig (97%), Chicken (90%), Chimpanzee (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha
基因别名: 5330430M07Rik; ARHGDIA; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1
UniProt ID: (Human) P52565, (Mouse) Q99PT1
Entrez Gene ID: (Human) 396, (Mouse) 192662
movement of cell or subcellular component
negative regulation of cell adhesion
regulation of Rho protein signal transduction
negative regulation of apoptotic process
positive regulation of GTPase activity
negative regulation of axonogenesis
positive regulation of axonogenesis
regulation of small GTPase mediated signal transduction
semaphorin-plexin signaling pathway
regulation of actin cytoskeleton reorganization
Rho protein signal transduction
regulation of cell shape
negative regulation of cell migration
regulation of protein localization
regulation of catalytic activity
cellular response to mechanical stimulus
