Invitrogen

Rhodopsin Polyclonal Antibody

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Rhodopsin Antibody (PA1-729) in WB

Western blot was performed using Anti-Rhodopsin Polyclonal Antibody (Product # PA1-729)and a 39 kDa band along with high molecular weight glycosylated forms corresponding to Rhodopsin were observed across all the tissues tested except Mouse Heart and Liver which are reported to be negative. Whole cell extracts (30 µg lysate) of Mouse Retina (Lane 1), Rat Retina (Lane 2), Rat Eye (Lane 3), Mouse Eye (Lane 4), Mouse Heart (Lane 5), and Mouse Liver (lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). R... View More

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产品信息

PA1-729

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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产品规格

种属反应

Bovine, Sheep

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C S(338) K T E T S Q V A P A(348)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2178960

产品详细信息

PA1-729 detects rhodopsin from bovine and sheep retina samples. This antibody does not detect rhodopsin from ovine samples

PA1-729 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.

The PA1-729 immunizing peptide (Cat. # PEP-174) corresponds to amino acid residues 338-348 from bovine rhodopsin. This peptide is available for use in neutralization and control experiments.

靶标信息

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

仅用于科研。不用于诊断过程。未经明确授权不得转售。