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Rhodopsin Antibody (MA5-45245) in IHC
Immunohistochemistry analysis using retina. Samples were incubated with Rhodopsin monoclonal antibody (Product # MA5-45245) at 1:1,000, followed by FITC Goat Anti-Mouse (green). Counterstain used was DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.
产品信息
MA5-45245
应用
建议稀释比
已发表文章
产品规格
种属反应
Amphibian,
Avian,
Bovine,
Fish,
Human,
Mouse,
Shark
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4D2
抗原
Bovine Rhodopsin
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931699
产品详细信息
1 µg/mL of MA5-45245 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
靶标信息
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4
基因别名: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Bovine) P02699, (Human) P08100, (Mouse) P15409
Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Mouse) 212541
G-protein alpha-subunit binding
opsin binding
guanyl-nucleotide exchange factor activity
11-cis retinal binding
protein binding
G-protein coupled photoreceptor activity
zinc ion binding
identical protein binding
arrestin family protein binding
G-protein coupled receptor activity
retinal binding
metal ion binding
signal transducer activity
photoreceptor activity
spectrin binding
G-protein coupled receptor
protein phosphorylation
visual perception
phototransduction, visible light
response to light stimulus
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
photoreceptor cell maintenance
retina development in camera-type eye
retinoid metabolic process
G-protein coupled receptor signaling pathway
red, far-red light phototransduction
regulation of rhodopsin mediated signaling pathway
signal transduction
phototransduction
detection of light stimulus
response to stimulus
sensory perception of light stimulus
cellular response to light stimulus
