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S100A6 Antibody (MA5-32511) in ICC/IF
Immunofluorescence analysis of S100A6 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with S100A6 Recombinant Rabbit Monoclonal Antibody (JF0976) (Product # MA5-32511) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
产品信息
MA5-32511
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JF0976
抗原
Recombinant full length protein of Human S100 alpha 6 aa 1-90
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809788
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 5B10; calcium binding protein A6 (calcyclin); Calcyclin; Growth factor-inducible protein 2A9; MLN 4; PRA; Prolactin receptor-associated protein; Protein S100-A6; S100 calcium-binding protein A6; S100 calcium-binding protein A6 (calcyclin)
基因别名: 2A9; 5B10; CABP; CACY; CALCYCLIN; PRA; S100A6
UniProt ID: (Human) P06703, (Mouse) P14069, (Rat) P05964
Entrez Gene ID: (Human) 6277, (Mouse) 20200, (Rat) 85247
