Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 11
S100A9 Antibody (MA5-32543) in ICC/IF
Immunofluorescence analysis of S100A9 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with S100A9 Recombinant Rabbit Monoclonal Antibody (JF096-8)(Product # MA5-32543) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More
产品信息
MA5-32543
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JF096-8
抗原
Synthetic peptide within Human S100A9 aa 1-42
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809820
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Protein S100-A9; S100 A9; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)
基因别名: 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9
UniProt ID: (Human) P06702
Entrez Gene ID: (Human) 6280
cytokine production
leukocyte migration involved in inflammatory response
positive regulation of peptide secretion
regulation of translation
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
signal transduction
cell-cell signaling
astrocyte development
positive regulation of blood coagulation
positive regulation of cell growth
neutrophil chemotaxis
actin cytoskeleton reorganization
sequestering of zinc ion
chemokine production
peptidyl-cysteine S-trans-nitrosylation
defense response to bacterium
innate immune response
regulation of integrin biosynthetic process
positive regulation of inflammatory response
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
neutrophil aggregation
cellular oxidant detoxification
positive regulation of intrinsic apoptotic signaling pathway
