Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
SAE1 Antibody (200-401-913) in WB
Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of Rocklands purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kDa band present in 35 µg lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine ... View More
产品信息
200-401-913
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1.
偶联物
形式
Lyophilized
浓度
5 mg/mL
纯化类型
Protein A
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This purified antibody is directed against human SUMO Activating Enzyme E1 protein. A BLAST analysis was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%) bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
靶标信息
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2. form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: activator of SUMO1; FLJ3091; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme
基因别名: AOS1; HSPC140; SAE1; SUA1; UBLE1A
UniProt ID: (Human) Q9UBE0
Entrez Gene ID: (Human) 10055
