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Invitrogen
SAP102 Polyclonal Antibody
FIGURE: 1 / 12
SAP102 Antibody (PA5-29116) in ICC/IF
Immunofluorescence analysis of SAP102 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SAP102 Rabbit Polyclonal Antibody(Product # PA5-29116) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
PA5-29116
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 525 and 803 of Human SAP102
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546592
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain, rat brain.
Predicted reactivity: Mouse (99%), Rat (92%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three approximately 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: discs large homolog 3; discs, large homolog 3; Disks large homolog 3; Neuroendocrine-DLG; protein phosphatase 1, regulatory subunit 82; PSD-95/SAP90-related protein 1; SAP-102; SAP102; Synapse-associated protein 102; XLMR
Gene Aliases: DLG3; Dlgh3; KIAA1232; mKIAA1232; MRX; MRX90; NEDLG; PPP1R82; SAP102; XLMR
UniProt ID: (Human) Q92796, (Rat) Q62936, (Mouse) P70175
Entrez Gene ID: (Pig) 100516124, (Human) 1741, (Rat) 58948, (Mouse) 53310
guanylate kinase activity
protein binding
protein C-terminus binding
ligand-gated ion channel activity
kinase binding
phosphatase binding
protein phosphatase binding
PDZ domain binding
ubiquitin protein ligase binding
ionotropic glutamate receptor binding
protein domain specific binding
scaffold/adaptor protein
establishment of planar polarity
chemical synaptic transmission
nervous system development
negative regulation of cell proliferation
negative regulation of phosphatase activity
ion transmembrane transport
receptor clustering
establishment or maintenance of epithelial cell apical/basal polarity
GMP metabolic process
GDP metabolic process
receptor localization to synapse
nucleotide phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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