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SAP97 Antibody (PA1-044) in WB
Western blot analysis was performed on membrane extracts (30 µglysate) of HeLa (Lane 1), and MCF-7 (Lane 2). The blots were probed with Rabbit Anti-SAP97 Polyclonal Antibody (Product # PA1-044, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to SAP97 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-... View More
产品信息
PA1-044
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2092021
产品详细信息
PA1-044 detects synapse-associated protein 97 (SAP97) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.
PA1-044 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain homogenate.
The PA1-044 immunogen is a synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97. This sequence is completely conserved in rat and mouse. PA1-044 immunizing peptide (Cat. # PEP-022) is available for use in neutralization and control experiments.
靶标信息
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97
基因别名: dJ1061C18.1.1; DLG1; DLGH1; hdlg; SAP-97; SAP97
UniProt ID: (Human) Q12959, (Rat) Q62696
Entrez Gene ID: (Human) 1739, (Rat) 25252
guanylate kinase activity
phosphoprotein phosphatase activity
protein binding
protein C-terminus binding
cytoskeletal protein binding
ligand-gated ion channel activity
potassium channel regulator activity
protein kinase binding
phosphatase binding
mitogen-activated protein kinase kinase binding
protein complex scaffold
ionotropic glutamate receptor binding
ion channel binding
L27 domain binding
cadherin binding involved in cell-cell adhesion
PDZ domain binding
ureteric bud development
branching involved in ureteric bud morphogenesis
immunological synapse formation
endothelial cell proliferation
lens development in camera-type eye
T cell cytokine production
actin filament organization
cell adhesion
chemical synaptic transmission
nervous system development
protein localization
positive regulation of cell proliferation
single organismal cell-cell adhesion
peristalsis
positive regulation of actin filament polymerization
cortical actin cytoskeleton organization
membrane raft organization
regulation of myelination
activation of protein kinase activity
regulation of protein localization
T cell activation
negative regulation of T cell proliferation
regulation of membrane potential
amyloid precursor protein metabolic process
receptor clustering
positive regulation of potassium ion transport
establishment or maintenance of epithelial cell apical/basal polarity
negative regulation of mitotic cell cycle
nucleotide phosphorylation
reproductive structure development
embryonic skeletal system morphogenesis
tissue morphogenesis
smooth muscle tissue development
negative regulation of epithelial cell proliferation
negative regulation of protein kinase B signaling
hard palate development
bicellular tight junction assembly
protein localization to plasma membrane
positive regulation of establishment of protein localization to plasma membrane
receptor localization to synapse
negative regulation of transcription from RNA polymerase II promoter
protein dephosphorylation
mitotic cell cycle checkpoint
establishment or maintenance of cell polarity
regulation of cell shape
viral process
astral microtubule organization
ion transmembrane transport
cellular protein complex localization
cortical microtubule organization
GMP metabolic process
GDP metabolic process
establishment of centrosome localization
negative regulation of ERK1 and ERK2 cascade
regulation of NIK/NF-kappaB signaling
regulation of sodium ion transmembrane transport
negative regulation of p38MAPK cascade
