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SAPAP1 Antibody (PA5-119751) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of GKAP in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with SAPAP1 Polyclonal Antibody (Product # PA5-119751) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119751
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within human GKAP aa 380-430
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2913323
产品详细信息
Positive Control: Human brain tissue cell lysates, PC-12, N2A, SHG-44, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue, Daudi.
Subcellular Location: Cell junction, Cell membrane, Membrane, Synapse.
靶标信息
Part of the postsynaptic scaffold in neuronal cells. Target binds the guanylate kinase-like domain of the PSD-95 family of synaptic ion channel clustering proteins; may play a role in protein-protein interactions at the synaptic junction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DAP-1; DAP-1 beta; discs large homolog associated protein 1; discs, large (Drosophila) homolog-associated protein 1; Disks large-associated protein 1; guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; PSD-95 binding protein; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; rGKAP; SAP90/PSD-95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP1
基因别名: 4933422O14Rik; 9630002F18; AI845682; AI848168; BB075781; D17Bwg0511e; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1; DLGAP1A; DLGAP1B; GKAP; GKAP/SAPAP; GKAP/SAPAP1; GKPA/SAPAP; Kiaa4162; mKIAA4162; rGKAP; SAPAP1
UniProt ID: (Human) O14490, (Mouse) Q9D415, (Rat) P97836
Entrez Gene ID: (Human) 9229, (Mouse) 224997, (Rat) 65040
